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has gloss | eng: Fluorescence Loss in Photobleaching, or FLIP, is a technique in fluorescence microscopy which can be used to examine the movement or diffusion of molecules inside cells or membranes. Typically a cell membrane is labelled with a fluorescent dye, and a specific area of the labeled membrane is bleached using the beam from a confocal laser scanning microscope. The fluorescence intensity from that region of the membrane is measured over time. Motion of fluorescent molecules into and along the membrane slowly restores the fluorescence in the bleached region, while depleting the fluorescence in other regions (by exchange of bleached for unbleached fluorophores). |
lexicalization | eng: Fluorescence loss in photobleaching |
instance of | c/Biochemistry methods |
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