| has gloss | eng: __NOTOC__ SILAC (Stable isotope labeling with amino acids in cell culture) is a mass spectrometry-based technique developed to detect differences in protein abundance between two (or more) samples (Ong et al., 2002; Zhu et al., 2002). It is one of the most popular methods for quantitative proteomics. Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing normal amino acids. In contrast, the growth medium of the second cell population contains amino acids labeled with stable (non-radioactive) heavy isotopes. For example, the medium can contain arginine labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). When the cells are growing in this medium, they incorporate the heavy arginine into all of their proteins. |
