e/Tandem Affinity Purification

Information
has gloss	eng: The Tandem Affinity Purification (TAP) is a technique for studying protein-protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The protein of interest with the TAP tag first binds to beads coated with IgG, the TAP tag is then broken apart by an enzyme, and finally a different part of the TAP tag binds reversibly to beads of a different type. After the protein of interest has been washed through two affinity columns, it can be examined for binding partners.
lexicalization	eng: Tandem affinity purification
instance of	e/Protein-protein interaction assays

Meaning
French
has gloss	fra: La méthode TAP est une méthode de purification de complexes protéiques basée sur lutilisation de protéines chimériques portant une étiquette composée de deux domaines daffinité (historiquement CBP et protéine A). Le principe de la méthode repose sur lutilisation succesives de deux colonnes daffinité, les complexes étant détachées de la première colonne par un clivage protéolytique, et par compétition de la seconde colonne.
lexicalization	fra: méthode TAP

Media
media:img	Taptag simple.png