e/Bimolecular fluorescence complementation

Information
has gloss	eng: Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components of the same macromolecular complex. Proteins that are postulated to interact are fused to unfolded complementary fragments of a fluorescent reporter protein and expressed in live cells. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. This fluorescent signal can be detected and located within the cell using an inverted fluorescence microscope that allows imaging of fluorescence in cells. In addition, the intensity of the fluorescence emitted is proportional to the strength of the interaction, with stronger levels of fluorescence indicating close or direct interactions and lower fluorescence levels suggesting interaction within a complex.
lexicalization	eng: Bimolecular Fluorescence Complementation
instance of	c/Biological techniques and tools

Meaning
German
has gloss	deu: Die Bimolekulare Fluoreszenzkomplementation (engl. bimolecular fluorescence complementation, BiFC) ist ein Verfahren der Molekularbiologie zum Nachweis von Protein-Protein-Interaktionen. Das Verfahren basiert auf der Komplementation zweier nicht fluoreszierender Fragmente eines fluoreszierenden Proteins, wie beispielsweise grün fluoreszierendes Protein (GFP). Durch Zusammenlagerung der beiden Fragmente wird ein intakter, fluoreszierender Komplex erzeugt. Das Verfahren der bimolekularen Fluoreszkomplementation wurde maßgeblich von Tom Kerppola und Mitarbeitern entwickelt .
lexicalization	deu: bimolekulare Fluoreszenzkomplementation

Media
media:img	BiFC (details).png
media:img	BiFC.png
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media:img	Flow2.jpg
media:img	Fluorescence resonance energy transfer.jpg
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