| Information | |
|---|---|
| has gloss | eng: The Single Cell Gel Electrophoresis assay (also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first described by Singh et al. in 1988. It has since gained in popularity as a standard technique for evaluation of DNA damage/repair, biomonitoring and genotoxicity testing. It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended lysed cells. This is followed by visual analysis with staining of DNA and calculating fluorescence to determine the extent of DNA damage. This can be performed by manual scoring or automatically by an imaging software. |
| lexicalization | eng: comet assay |
| instance of | c/Chemical tests |
| Meaning | |
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| Arabic | |
| has gloss | ara: اختبار المذنب |
| lexicalization | ara: اختبار المذنب |
| German | |
| has gloss | deu: Der Comet Assay (auch Einzelzellgelelektrophorese genannt) ist eine Technik, durch die es ermöglicht wird, DNA-Schädigungen in einzelnen Zellen festzustellen. Entwickelt wurde der Assay 1984 von Östling und Johanson zum Nachweis von DNA-Doppelstrangbrüchen. Mit der Weiterentwicklung durch Singh im Jahre 1988 konnten durch die Verwendung von basischen Puffern zusätzlich auch DNA-Einzelstrangbrüche festgestellt werden. |
| lexicalization | deu: Comet Assay |
| Italian | |
| has gloss | ita: Il test della cometa noto anche come elettroforesi su singola cellula è un test di mutagenesi per l'identificazioni di danni al DNA in una cellula (solitamente di mammifero). Il test è conosciuto anche con le dizioni inglesi comet assay, Single Cell Gel Electrophoresis e il suo acronimo SCGE. |
| lexicalization | ita: test della cometa |
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